| 詳細描述： |
<strong>Gene Name Synonym:</strong><br /> CD340; ENV; ENVW; ERVWE1; HER-2; HER-2/neu; HER2; HERV-7q;HERV-W-ENV; HERV7Q; HERVW; HERVWENV; MLN 19; MLN19; NEU;NGL; TKR1 <strong>純度：</strong>> 90 % as determined by SDS-PAGE<br /> <strong>內毒素</strong>：< 1.0 EU per μg of the protein as determined by the LAL method<br /> <strong>表達宿主</strong>：HEK293 Cells<br /> <strong>種屬：</strong>Human<br /> <strong>預測 </strong>：N 端Thr 23<br /> <strong>分子量</strong>：The recombinant human ErbB2 comprises 641 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, rh ErbB2 migrates as an approximately 100-110 kDa protein in SDS-PAGE under reducing conditions.<br /> <strong>生物活性</strong><br /> 1. Measured by its binding ability in a functional ELISA.<br /> Immobilized human Erbb2 at 2.5 μg/ml (100 μl/well) can bind Herceptin with a linear range of 1.28-32 ng/ml.<br /> 2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.2-1 μg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.<br /> 3. Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant (His Tag) (Cat: 10004-H08H) bound to Pertuzumab was 0.1 nM.<br /> <strong>蛋白構建：</strong><br /> A DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ErbB2 (NP_004439.2) was fused with the polyhistidine tag at the C-terminus.<br /> <strong>緩沖液：</strong><br /> Lyophilized from sterile PBS, pH 7.4<br /> Please contact us for any concerns or special requirements.<br /> Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.<br /> Please refer to the specific buffer information in the hard copy of CoA.<br /> <strong>運輸方式：</strong><br /> In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.<br /> Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.<br /> <strong>穩(wěn)定性 & 儲存條件：</strong><br /> Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃<br /> Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.<br /> <strong>復溶：</strong><br /> A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.<br /> |
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